High resolution 3D biomechanical mapping of embryos with Optical Coherence Elastography

Christian Zevallos Delgado
SEC 206
Healthy development of embryos depends on several critical biomechanical processes, such as
neurulation and cardiovascular development. Understanding the structural modifications and changes
in stiffness during embryo development is important for understanding the etiology of various
congenital diseases, such as anencephaly or spina bifida. The noninvasively high-resolution 3D
mapping of the biomechanical properties of the embryos was done using reverberant optical
coherence elastography (Rev-OCE) without any exogenous contrast agents. Rev-OCE measurements
were performed in both murine and zebrafish embryos to showcase its capability to map the stiffness
of commonly used models of disease. Murine embryos were dissected from CD1 mice at different
gestational days, and the zebrafish embryos were at different hours and days post fertilization. Rev-
OCE imaging was performed using a phase-sensitive optical coherence tomography (PhS-OCT)
system, the wave generation of wave was done using a piezoelectric bender in the samples. The
bender vibrated and generated randomly oriented shear waves in the samples, which were detected
by the PhS-OCT system. The results show clear spatial distribution of stiffness in the embryos. For
example, the spinal region of the murine embryos was stiffer than other tissues, and in the zebrafish
embryos, the head and swim bladder were stiffer. Embryonic elasticity could provide valuable insight
about critical embryonic developmental process and etiology of various congenital defects.